Handi Priambodo,1 Suradi,1 Reviono,1 Yusup S Sutanto,1 Ana Rima,1 Harsini,1 Yuyun Rindiastuti,2 Abdurahman Laqif,3 Indah Julianto2
1Departemen Pulmonologi dan Kedokteran Respirasi, Fakultas Kedokteran Universitas Sebelas Maret, RSUD Dr. Moewardi, Surakarta
2Departemen Kulit dan Kelamin, Fakultas Kedokteran Universitas Sebelas Maret, RSUD Dr. Moewardi, Surakarta
3Departemen Obstetri dan Ginekologi, Fakultas Kedokteran Universitas Sebelas Maret, RSUD Dr. Moewardi, Surakarta
Abstract
Background: Cigarette smoking causes pulmonary inflammation and apoptosis implicated in COPD. MSC secreting factors plays crucial role in tissue regeneration. Using MSC conditioned media (MSC-CM) might be an alternativeto MSC transplantation. Conditioned media was obtained from hypoxic 3D culture of amniotic membrane MSC. This study aimed to test the effect of administration of MSC-CM in inhibiting apoptosis in cigarette smoke lung damage. The aim of this study is to test the effect of administration of MSC-CM in inhibiting apoptosis in cigarette smoke induced lung damage
Methods: Research was conducted in 5 months from February to Juni 2015 and under ethical clearance approval. To accomplish this study, 30 mice were exposed to cigarrete smoke for 12 weeks, 15 mice followed by MSC-CM administration and others with unconditioned media, mean linier intercept (mLi) and Caspase-3 were measured from lung histophatology. In vitro study using fibroalveolar cell exposed to cigarette smoke exctract (2%CSE) followed with MSC-CM was conducted to confirm effect of MSC-CM by MTT assay and VEGF level measurement.
Result: This research revealed that MSC-CM inhibit lung damage confirmed with significantly lower mLi, while inhibit pulmonary cell apoptosis with significantly lower Caspase-3 level in mice administered with MSC-CM (p=0.00). In vitro study provides the evidence that MSC-CM promote fibroalveolar proliferation (MTT assay; p=0.00), while mediates anti-apoptosis effect which partly depends on up regulating of VEGF (VEGF level of MSC-CM group=1082.06 pg/ml; control group 261.027 pg/ml)
Conclusion: MSC-CM has therapeutic potential in cigarette smoke induced lung damage in mice by inhibiting cell apoptosis as well as upregulating VEGF. (J Respir Indo. 2016; 36: 249-56)
Keywords: Cigarette smoke, apoptosis, stem cell conditioned media