Interleukin-10-1082G/A Gene Polymorphism as a Potential Host Susceptibility Factor in Patients with Multidrug Resistant Tuberculosis

· Biomolecular, Journal, Lung Infection

Novita Eva Sawitri,1 Reviono,1 Suradi,1 Afiono Agung Prasetyo2

1Bagian Pulmonologi dan Kedokteran Respirasi, Fakultas Kedokteran Universitas Sebelas Maret, RSUD Dr. Moewardi, Surakarta

2Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Sebelas Maret, Surakarta


Background: This study was conducted to determine the association between polymorphism of interleukin (IL)-10 -1082G/A gene on susceptibility of multidrug resistant tuberculosis (MDR-TB). Polymorphism of IL-10-1082G/A as a single nucleotide polymorphism of IL-10 gene affects IL-10 cytokine production. The IL-10 cytokine is a potent immunoregulator to TB infection. Polymorphism of IL-10 -1082G/A gene is susceptible for TB.

Methods: Socio-epidemiological data and blood specimens were collected from 30 MDR-TB subjects, 29 non MDR-TB subjects and 34 healthy subjects. Blood specimens were analyzed for IL-10-1082G/A gene polymorphism by PCR. This study was a cross-sectional study conducted in Dr Moewardi Hospital Surakarta on November and December 2014. We compared the frequencies of IL-10-1082G/A, IL-10-1082A/A, and IL-10-1082G/G genotypes among groups.

Results: Genotype of IL-10 -1082 A/A was more frequent in MDR-TB group 33.3% (10/30) compares to non MDR-TB group 10.3% (3/29) and healthy group 8.8% (3/34). The frequency of IL-10-1082G/A genotype in MDR-TB group 66.7% (20/30), TB group 89.7% (26/29), and healthy group 91.2% (31/34). The frequency of IL-10-1082A/A and IL-10 -1082 G/A genotypes were significantly different between these groups (p=0.051). Polymorphism of IL-10-1082G/A gene is associated to MDR-TB. There was no IL-10-1082G/G find in this study.
Conclusion: Polymorphism of IL-10-1082G/A gene is associated to susceptibility of MDR-TB in this study. (J Respir Indo. 2016; 36: 1-10)

Keywords: MDR-TB, susceptibility, polymorphism of IL-10-1082G/A gene.

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