Expression of Protein Telomerase on Paraffin Block Preparations of Non-Small Cell Lung Cancer

· Biomolecular, Thoracic Oncology

Heidy Agustin1, Elisna Syahruddin1, Faisal Yunus1, Endarjo S2
1Departemen Pulmonologi dan Kedokteran Respirasi
Fakultas Kedokteran Universitas Indonesia, RSUP Persahabatan, Jakarta
2Departemen Patologi Anatomi, RSUP Persahabatan, Jakarta

Background: Lung cancer is a leading cause of death from cancer group. Prooncogenes and tumor suppressor genes role are separately known as k-ras, p53 and myc. Other factors are telomere and telomerase enzyme. Telomerase known as a malignancy marker also an prognostic and therapy indicator.
Methods: A cross sectional study held in Pulmonology and Respiration Departement, Persahabatan Hospital and Dharmais Cancer Hospital, Jakarta from October 2007-July 2008. Thirty three subjects who had undergone surgery involved in this study. All of subjects diagnosed as non small cell lung cancer (NSCLC). Each parafin block of subjects got immunohistochemistry stained with antimouse monoclonal antibody telomerase. Subjects or their family contacted for providing survival data. All of data collected analyzed with anova one way, Kruskall-wallis and Kaplan Meyer for survival analysis.
Results: All of parafin blocks expressed telomerase. There were no significant correlation between telomerase protein expression with age, gender, smoking history and histopatology type. Telomerase protein expression had significant correlation with N status but not in staging, T and M status. Telomerase protein expression is not a prediction factor for survival. Median survival rate was 14 months and 1 year survival rate was 63.7%.
Conclusion: This research describes telomerase expression of NSCLC. All of NSCLS cases express telomerase and there are no significant correlation between variable and telomerase expression. (J Respir Indo. 2017; 37: 225-31)

Keywords: Kanker paru, KPKBSK, telomerase

Download article here